Journal: JCI Insight

Article Title: Hnf4a deletion in the mouse kidney phenocopies Fanconi renotubular syndrome

doi: 10.1172/jci.insight.97497

Figure Lengend Snippet: (A) In nascent S-shaped body (SSB), Hnf4a is not detected. Representative image of n = 3. Scale bar: 25 μm. (B) Hnf4a is expressed in a subset of Jag1+ cells in the medial segment of the elongated SSB. Scale bar: 25 μm. Image is representative of n = 3. (C) Hnf4a is detected in presumptive proximal tubule (PT) cells and differentiated PT cells. Presumptive PT cells show weak Lotus tetragonolobus lectin (LTL) staining and are located more cortically, where early stages of nephrogenesis occur. Differentiated PT cells show strong LTL staining and are located closer to the medullary region. Image is representative of n = 3. Scale bar: 100 μm.

Article Snippet: We used the primary antibodies for GFP (1:500, Aves, GFP-1020), Jag1 (1:20, Developmental Studies Hybridoma Bank [DSHB], TS1.15H), Wt1 (1:100, Santa Cruz Biotechnology Inc., sc-7385), Biotin-LTL (1:500, Vector Laboratories, B-1325), FITC-LTL (1:200, Vector Laboratories, FL-1321), Slc12a1 (1:500, Proteintech, 18970-1-AP), Slc12a3 (1:300, MilliporeSigma, HPA028748), Hnf4a (1:1,000, Santa Cruz Biotechnology Inc., sc-8987X; or 1:500, Abcam, ab41898), Lrp2 (1:100, Santa Cruz Biotechnology Inc., sc-515772), and Ass1 (1:500, Proteintech, 16210-1-AP).

Techniques: Staining

Journal: Experimental and Therapeutic Medicine

Article Title: Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF-β1/Smad/miR-192 signaling pathway

doi: 10.3892/etm.2019.7887

Figure Lengend Snippet: Effects of ASI on miR-192 expression and RMC proliferation. (A) miR-192 expression exhibited by the LG and HG treatment groups. *P<0.05 vs. the LG group. (B) miR-192 expression following treatment with ASI at 0, 10, 20, 50, and 100 µg/ml. *P<0.05 vs. 0 µg/ml ASI group and **P<0.01 vs. 0 µg/ml ASI group. (C) Cell proliferation following treatment with LG and HG. *P<0.05 vs. the LG group. (D) Cell proliferation following treatment with ASI at 0, 10, 20, 50, and 100 µg/ml at 0, 24, 48 and 72 h. *P<0.05 vs. 0 µg/ml ASI group and **P<0.01 vs. 0 µg/ml ASI group. Data are presented as the mean ± standard deviation (n=3). Each bar represents the mean of three independent experiments, and experiments were performed in triplicate. ASI, astragaloside IV; miR, microRNA; RMC, rat mesangial cells; LG, low glucose; HG, high glucose; OD, optical density.

Article Snippet: Rat mesangial cells (RMCs; cat. no. CRL-2573) were obtained from the American Type Culture Collection.

Techniques: Expressing, Standard Deviation

Journal: Experimental and Therapeutic Medicine

Article Title: Astragaloside IV inhibits excessive mesangial cell proliferation and renal fibrosis caused by diabetic nephropathy via modulation of the TGF-β1/Smad/miR-192 signaling pathway

doi: 10.3892/etm.2019.7887

Figure Lengend Snippet: Effects of ASI on the TGF-β1/Smad signaling pathway in RMCs. (A) Reverse transcription-quantitative PCR analysis of TGF-β1, Smad3, col1, α-SMA and Smad7 mRNA in LG, HG and HG + ASI groups. (B) Western blot and (C) subsequent quantification and analysis of TGF-β1, Smad3, col1, α-SMA and Smad7 mRNA in LG, HG and HG + ASI groups. Data are presented as the mean ± standard deviation (n=3). Each bar represents the mean of three independent experiments, and experiments were performed in triplicate. *P<0.05 vs. LG group; # P<0.05 vs. HG group. ASI, astragaloside IV; TGF-β1, transforming growth factor-β1; RMC, rat mesangial cells; col1, collagen type 1; α-SMA, α-smooth muscle actin; HG, high glucose.

Article Snippet: Rat mesangial cells (RMCs; cat. no. CRL-2573) were obtained from the American Type Culture Collection.

Techniques: Reverse Transcription, Real-time Polymerase Chain Reaction, Western Blot, Standard Deviation

Journal: Frontiers in Pharmacology

Article Title: The Role and Mechanism of Histone Deacetylases in Acute Kidney Injury

doi: 10.3389/fphar.2021.695237

Figure Lengend Snippet: HDAC alterations in AKI.

Article Snippet: , Bilateral I/R (mild) , Male Sprague Dawley rat kidney , Western blot , ↑SIRT1 , .

Techniques: Western Blot, Real-time Polymerase Chain Reaction, Immunohistochemistry

Journal: eLife

Article Title: The spatial separation of processing and transport functions to the interior and periphery of the Golgi stack

doi: 10.7554/eLife.41301

Figure Lengend Snippet:

Article Snippet: Cell line ( Rattus norvegicus ) , Normal rat kidney (NRK) fibroblast cell , ATCC , ATCC: CRL-1570; RRID: CVCL_2144 , .

Techniques: Recombinant, Plasmid Preparation, Antibody Labeling, Software